UBE2K anticorps (C-Term)

N° du produit ABIN374290

Cet anticorps Chèvre Polyclonal détecte spécifiquement UBE2K dans WB. Il présente une réactivité envers Humain, Souris, Chien, Boeuf (Vache) et Porc.

Aperçu rapide pour UBE2K anticorps (C-Term) (ABIN374290)

Antigène: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Reactivité: Humain, Souris, Chien, Boeuf (Vache), Porc

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp UBE2K est non-conjugé

Application: Western Blotting (WB)

Détail du produit anti-UBE2K anticorps

Épitope: C-Term

Séquence: C-SWDVETATEL LLSN

Specificité: This antibody recognizes UBE2K/HIP2 (C-term). It is expected to recognise all reported isoforms.

Réactivité croisée (Details): Species reactivity (expected):Mouse, Canine (Dog), Pig and Cow.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogène: Peptide from C Terminus of the protein sequence according to NP_005330.1, NP_001104582.1, NP_001104583.1. Genename: UBE2K

Information d'application

Indications d'application: Peptide ELISA: Limit Dilution: 1/2000. Western blot: 1-3 μg/mL. Approx 24 kDa band observed in Jurkat cell lysates (CalculatedMW of 22.4 kDa according to NP_005330).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Avoid repeated freezing and thawing.

Stock: -20 °C

Stockage commentaire: Store the antibody undiluted at (in aliquots) at -20 °C.

Détails sur UBE2K

Antigène: UBE2K (Ubiquitin-Conjugating Enzyme E2K (UBE2K))

Autre désignation: HIP2 / UBE2K

Sujet: The protein encoded by this gene belongs to the ubiquitin-conjugating enzyme family. This protein interacts with RING finger proteins, and it can ubiquitinate huntingtin, the gene product for Huntington's disease. Known functions for this protein include a role in aggregate formation of expanded polyglutamine proteins and the suppression of apoptosis in polyglutamine diseases, a role in the dislocation of newly synthesized MHC class I heavy chains from the endoplasmic reticulum, and involvement in foam cell formation. Multiple transcript variants encoding different isoforms have been identified for this gene. HIP2 belongs to the ubiquitin conjugating enzyme family. It binds selectively to a large region at the N terminus of huntingtin. This interaction is not influenced by the length of the huntingtin polyglutamine tract. This protein has been implicated in the degradation of huntingtin and suppression of apoptosis. Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short lived and abnormal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid bearing macrophages through ubiquitination and subsequent degradation of p53.Synonyms: E2(25K), E2-25K, HIP-2, Huntingtin-interacting protein 2, LIG, Ubiquitin carrier protein, Ubiquitin-conjugating enzyme E2 K, Ubiquitin-protein ligase

ID gène: 3093

NCBI Accession: NP_005330

UniProt: P61086